Structure of UvrABC excinuclease-UV-damaged DNA complexes studied by flow linear dichroism DNA curved by UvrB and UvrC
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چکیده
منابع مشابه
Incision of damaged versus nondamaged DNA by the Escherichia coli UvrABC proteins.
Incision of damaged DNA by the Escherichia coli UvrABC endonuclease requires the UvrA, UvrB, and UvrC proteins as well as ATP hydrolysis. This incision reaction can be divided into three steps: site recognition, preincision complex formation, and incision. UvrAB is able to execute the first two steps in the reaction while the addition of UvrC is required for the incision of DNA. This incision r...
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UV light-induced photoproducts are recognized and removed by the nucleotide-excision repair (NER) pathway. In humans, the UV-damaged DNA-binding protein (UV-DDB) is part of a ubiquitin E3 ligase complex (DDB1-CUL4A(DDB2)) that initiates NER by recognizing damaged chromatin with concomitant ubiquitination of core histones at the lesion. We report the X-ray crystal structure of the human UV-DDB i...
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The internal structure of DNA-CTAB and DNA-DDAB is investigated by a Small Angel X- ray Scattering(SAXS) instrument. Hexagonal packing of DNA was observed for DNA complex with CTAB, and for DDABcomplex is observed lamellar structure. Variations in the internal spacing and degree of long-range ordering aredependent on both surfactant type and concentrations of added salt. When we increased the a...
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Some differences between the structure of tumour and normal DNA were studied. An increase of the methylation degree of the tumour DNA was observed (by 42% as compared with normal DNA). Changes between normal and tumour DNA were found in the melting temperature (Tm), the melting interval (delta T) and the shape of the differential melting curves (DMC). In tumour DNA, two types of regions with ch...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1992
ISSN: 0014-5793
DOI: 10.1016/0014-5793(92)81448-u